Coding

Part:BBa_J72020:Design

Designed by: Jennifer Brophy   Group: Anderson Lab   (2010-07-15)


{Bmll50} in BBb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

Overlap Extension PCR (Wobble Construction)

Wobble construction of Bmll50 {rbs_at_10000.HA>}

Wobble mll50/mll51                (100, EcoRI/BamHI
Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll50         {rbs_at_10000.HA>}
----
mll50    Forward wobble for {rbs_at_10000.HA>} (Bmll50)
CCATAGAATTCATGAGATCTTAAGCACCCATAGAATACACACCTGGAGGAATAATGTCTTACC
mll51    Reverse wobble for {rbs_at_10000.HA>} (Bmll50)
CGTTAGGATCCCCCAGCGTAGTCTGGGACGTCGTATGGGTAAGACATTATTCCTC

References